Mikroekonomi sadono sukirno ebook. Practice dilution problems for the Bio 201 practical 1. Diagram a three step 10‐1 serial dilution. Serial Dilutions. A dilution series is a succession of step dilutions, each with the same dilution factor. To make a dilution series, use the following formulas.
Understanding how to make dilutions is an essential skill for biologist. This skill is used, for example, in making solutions, diluting bacteria, diluting antibodies, etc.
It is important to understand the following: - how to do the calculations to set up the dilution - how to do the dilution optimally - how to calculate the final dilution Click on the links on the left for the dilution tutorials. The purpose of these tutorials is to review these concepts and provided practice problems to review before coming to lab. Created by: Cecilia Yu '07 Maintained by: CarolAnn Paul Date Created: July 1, 2004 Last Modified: August 25, 2004 Page Expires: August 6, 2006|.
Overall Dilution
Advantages of 'Serial Dilutions' This section is not a recipe for your experiment. It explains some principles for designing dilutions that give optimal results.
Once you understand these principles, you will be better able to design the dilutions you need for each specific case. Often in experimental work, you need to cover a range of concentrations, so you need to make a bunch of different dilutions. For example, you need to do such dilutions of the standard IgG to make the standard curve in ELISA, and then again for the unknown samples in ELISA. You might think it would be good to dilute 1/2, 1/3, 1/10, 1/100. These seem like nice numbers. There are two problems with this series of dilutions. • The dilutions are unnecessarily complicated to make.
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You need to do a different calculation, and measure different volumes, for each one. It takes a long time, and it is too easy to make a mistake. • The dilutions cover the range from 1/2 to 1/100 unevenly. In fact, the 1/2 vs.
1/3 dilutions differ by only 1.5-fold in concentration, while the 1/10 vs. 1/100 dilutions differ by ten-fold.
If you are going to measure results for four dilutions, it is a waste of time and materials to make two of them almost the same. And what if the half-maximal signal occurs between 1/10 and 1/100? You won't be able to tell exactly where it is because of the big space between those two. Serial dilutions are much easier to make and they cover the range evenly.